Quantitect Reverse Transcription Kit

The kit is ideal for generating cDNA archives. Qiagen QuantiTect Reverse Transcription Kit 50 x 20L rxns RNA Template Sample Two-step cDNA Production Genomic DNA Digestion Reaction Type For Fast cDNA Synthesis Enabling Sensitive Real-time Two-step RT-PCR for Gene Expression Analysis Includes 100L 7x gDNA Wipeout Buffer 50L Quantiscript Reverse Transcriptase 200L 5x Quantiscript RT Buffer 50L RT Primer Mix 19mL RNase-free Water.

Quantitect Reverse Transcription Kit Qiagen Online Shop

When used in combination with the QuantiTect Reverse Transcription Kit and QuantiTect Primer Assays the QuantiTect SYBR Green PCR Kit delivers sensitive and reliable results see figure Specific and sensitive quantification.

Quantitect reverse transcription kit. The QuantiTect Reverse Transcription Kit Qiagen Melbourne VIC Australia was used to reverse-transcribe up to 1000 ng total RNA. QuantiTect SYBR Green PCR Kits are compatible with all available real-time cyclers including instruments from Applied Biosystems Bio-Rad Cepheid Eppendorf Roche and Agilent. Detection of reference gene and up to 3 targets in the same tube.

GDNA Wipeout Buffer Quantiscript Reverse Transcriptase Quantiscript RT Buffer RT Primer Mix and RNase-Free Water 1KT Catalog No. QuantiTect Reverse Transcription Kit 50 gDNA Wipeout Buffer 7x. The combination of a hot start and a unique PCR buffer system in the ready-to-use master mix ensures highly sensitive and reliable.

Qiagen QuantiTect Reverse Transcription Kit 400 x 20L rxns RNA Template Sample Two-step cDNA Production Genomic DNA Digestion Reaction Type For Fast cDNA Synthesis Enabling Sensitive Real-time Two-step RT-PCR for Gene Expression Analysis Includes 800L 7 x gDNA Wipeout Buffer 400L Quantiscript Reverse Transcriptase 16mL 5x Quantiscript RT Buffer 400L RT Primer Mix 8 x. I am about to use the quantitect reverse transcription kit which describes that Incubate for 15 min at 42C. CDNA 2 µL was amplified by Quantitative real-time PCR on the Rotor-Gene Q qPCR instrument Qiagen with 10 µl reactions using the SensiFAST SYBR No-Rox Kit Bioline.

Qiagen QuantiTect Reverse Transcription Kit 400 x 20L rxns RNA Template Sample Two-step cDNA Production Genomic DNA Digestion Reaction Type For Fast cDNA Synthesis Enabling Sensitive Real-time Two-step RT-PCR for Gene Expression Analysis Includes 800L 7 x gDNA Wipeout Buffer 400L Quantiscript Reverse Transcriptase 16mL 5x Quantiscript RT Buffer 400L RT Primer Mix 8 x 1. For fast cDNA synthesis for sensitive real-time two-step RT-PCR Fast and convenient procedure - synthesize cDNA and remove genomic DNA in only 20 minutes Effective genomic DNA removal - no need to design RNA-specific primers or. NC9392300 49166 Each.

33 50 1 reviews Write your own review. QuantiTect Reverse Transcription Kit by QIAGEN. Qiagen markets this kit specifically for qPCR which makes it very appealing to researchers doing real-time gene expression studies.

The combination of a hot start and a unique PCR buffer system in the ready-to-use master mix ensures highly sensitive qPCR on any real-time cycler without the need for optimization. QuantiTect Reverse Transcription KitはゲノムDNA除去を組み込んだcDNA合成を迅速かつ簡便な方法で実現します. QuantiTect Probe PCR Kits enable sensitive quantification of gDNA and cDNA targets by real-time PCR and two-step RT-PCR using sequence-specific probes.

QuantiTect Reverse Transcription Kit 50. Reactions can be scaled up to 100 µL to generate 10 µg of cDNA from a single reaction. Qiagens Quantitect Reverse Transcription Kit is designed to generate high quality cDNA which can be used for highly sensitive downstream applications such as real-time quantitative PCR qPCR.

In some rare cases eg if the RT-PCR product is longer than 200 bp or if analyzing. For 50 x 20uL rxn. United States of America.

ITaq Universal One-Step RT-qPCR Kits are a fast and convenient solution for real-time PCR using the powerful combination of iScript RNase H MMLV reverse transcriptase and hot-start iTaq DNA Polymerase in one reaction providing improved PCR efficiency wider dynamic range superior sensitivity and specificity and inhibitor tolerance without affecting performance. Reverse transcription of total RNA was carried out using the Quantitect kit Qiagen which includes a genomic DNA elimination step before RNA reverse transcription. QuantiTect SYBR Green PCR Kits Ref.

The QuantiTect SYBR Green PCR Kit is for use in gene expression analysis of cDNA targets and quantitative gDNA analysis. In contrast to kits from other suppliers the QuantiTect Reverse Transcription Kit provides high yields of cDNA template for real-time PCR analysis regardless of where the amplified target region is located on the transcript and provides greater sensitivity in the detection of low-abundance genes see figure Higher sensitivity in real-time two-step RT-PCR. QuantiTect Multiplex RT-PCR Kits enable reliable quantification of up to 5 RNA targets in a single tube by multiplex real-time one-step RT-PCR.

Quantiscript Reverse TranscriptaseQuantiscript RT Buffer画期的なRT Primer Mixにより迅速かつ効率的な逆転写反応に必要な全ての成分が提供されます. Downstream applications include real-time PCR standard PCR and microarrays. Q PCR experiments were performed in a 20-µL final volume containing 1 µL of cDNA 06 pmol of each primer and 10 µL of FastStart SYBR Green Master Mix Roche Diagnostics.

In contrast to kits from other suppliers the QuantiTect Reverse Transcription Kit provides high yields of cDNA template for real-time PCR analysis regardless of where the amplified target region is located on the transcript and provides greater sensitivity in the detection of low-abundance genes see figure Higher sensitivity in real-time two-step RT-PCR. The High Capacity cDNA Reverse Transcription Kit delivers extremely high-quality single-stranded cDNA from 002 to 2 µg total RNA.

Quantitect Sybr Green Rt Pcr Kit Qiagen Online Shop